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rabbit polyclonal anti glua3  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit polyclonal anti glua3

    Rabbit Polyclonal Anti Glua3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+glur3/Anti-GluR3+(GluA3)+(extracellular)+Antibody/pmc10504484-4-0-4
    Average 94 stars, based on 8 article reviews
    rabbit polyclonal anti glua3 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "GluK2 Q/R editing regulates kainate receptor signaling and long-term potentiation of AMPA receptors"

    Article Title: GluK2 Q/R editing regulates kainate receptor signaling and long-term potentiation of AMPA receptors

    Journal: iScience

    doi: 10.1016/j.isci.2023.107708


    Figure Legend Snippet:

    Techniques Used: Recombinant, Protein Extraction, Software

    Related Articles

    Saline:

    Article Title: AMPA Receptor Regulation at the mRNA and Protein Level in Rat Primary Cortical Cultures
    Article Snippet: Equal amounts of protein were applied to precast SDS polyacrylamide gels (4–12% NuPAGE Bis-Tris gels; Invitrogen) and the proteins were electrophoretically transferred to a Hybond-P PVDF Transfer Membrane (GE Healthcare, Waukesha, WI, USA) for 2 h at 1 mA/cm 2 membrane surface. .. The membranes were blocked for 60 min with 3% nonfat dry milk or 2% BSA in TBS-T (Tris-buffered saline with 0.1% Tween-20, Sigma-Aldrich) and then incubated overnight at 4°C in the blocking solution with the primary antibodies corresponding to either rabbit polyclonal anti-GluR1 (1∶200, Millipore), rabbit polyclonal anti-GluR2 (1∶2500, Millipore), rabbit polyclonal anti-GluR3 (1∶500, Alomone Labs Ltd) or rabbit polyclonal anti-GluR4 (1∶100, Millipore) with mouse monoclonal anti-GAPDH (1∶2500, Sigma-Aldrich) as a loading control. .. After 3 washes in TBS-T, the membranes were incubated for 1 h at room temperature with HRP- (Millipore) or AP-conjugated (Santa Cruz Biotechnology, Santa Cruz, CA, USA) secondary antibodies.

    Incubation:

    Article Title: AMPA Receptor Regulation at the mRNA and Protein Level in Rat Primary Cortical Cultures
    Article Snippet: Equal amounts of protein were applied to precast SDS polyacrylamide gels (4–12% NuPAGE Bis-Tris gels; Invitrogen) and the proteins were electrophoretically transferred to a Hybond-P PVDF Transfer Membrane (GE Healthcare, Waukesha, WI, USA) for 2 h at 1 mA/cm 2 membrane surface. .. The membranes were blocked for 60 min with 3% nonfat dry milk or 2% BSA in TBS-T (Tris-buffered saline with 0.1% Tween-20, Sigma-Aldrich) and then incubated overnight at 4°C in the blocking solution with the primary antibodies corresponding to either rabbit polyclonal anti-GluR1 (1∶200, Millipore), rabbit polyclonal anti-GluR2 (1∶2500, Millipore), rabbit polyclonal anti-GluR3 (1∶500, Alomone Labs Ltd) or rabbit polyclonal anti-GluR4 (1∶100, Millipore) with mouse monoclonal anti-GAPDH (1∶2500, Sigma-Aldrich) as a loading control. .. After 3 washes in TBS-T, the membranes were incubated for 1 h at room temperature with HRP- (Millipore) or AP-conjugated (Santa Cruz Biotechnology, Santa Cruz, CA, USA) secondary antibodies.

    Blocking Assay:

    Article Title: AMPA Receptor Regulation at the mRNA and Protein Level in Rat Primary Cortical Cultures
    Article Snippet: Equal amounts of protein were applied to precast SDS polyacrylamide gels (4–12% NuPAGE Bis-Tris gels; Invitrogen) and the proteins were electrophoretically transferred to a Hybond-P PVDF Transfer Membrane (GE Healthcare, Waukesha, WI, USA) for 2 h at 1 mA/cm 2 membrane surface. .. The membranes were blocked for 60 min with 3% nonfat dry milk or 2% BSA in TBS-T (Tris-buffered saline with 0.1% Tween-20, Sigma-Aldrich) and then incubated overnight at 4°C in the blocking solution with the primary antibodies corresponding to either rabbit polyclonal anti-GluR1 (1∶200, Millipore), rabbit polyclonal anti-GluR2 (1∶2500, Millipore), rabbit polyclonal anti-GluR3 (1∶500, Alomone Labs Ltd) or rabbit polyclonal anti-GluR4 (1∶100, Millipore) with mouse monoclonal anti-GAPDH (1∶2500, Sigma-Aldrich) as a loading control. .. After 3 washes in TBS-T, the membranes were incubated for 1 h at room temperature with HRP- (Millipore) or AP-conjugated (Santa Cruz Biotechnology, Santa Cruz, CA, USA) secondary antibodies.

    Control:

    Article Title: AMPA Receptor Regulation at the mRNA and Protein Level in Rat Primary Cortical Cultures
    Article Snippet: Equal amounts of protein were applied to precast SDS polyacrylamide gels (4–12% NuPAGE Bis-Tris gels; Invitrogen) and the proteins were electrophoretically transferred to a Hybond-P PVDF Transfer Membrane (GE Healthcare, Waukesha, WI, USA) for 2 h at 1 mA/cm 2 membrane surface. .. The membranes were blocked for 60 min with 3% nonfat dry milk or 2% BSA in TBS-T (Tris-buffered saline with 0.1% Tween-20, Sigma-Aldrich) and then incubated overnight at 4°C in the blocking solution with the primary antibodies corresponding to either rabbit polyclonal anti-GluR1 (1∶200, Millipore), rabbit polyclonal anti-GluR2 (1∶2500, Millipore), rabbit polyclonal anti-GluR3 (1∶500, Alomone Labs Ltd) or rabbit polyclonal anti-GluR4 (1∶100, Millipore) with mouse monoclonal anti-GAPDH (1∶2500, Sigma-Aldrich) as a loading control. .. After 3 washes in TBS-T, the membranes were incubated for 1 h at room temperature with HRP- (Millipore) or AP-conjugated (Santa Cruz Biotechnology, Santa Cruz, CA, USA) secondary antibodies.



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    Double labeling of rat cortical neurons at DIV5 and DIV26 (20×) with an antibody against the glial marker GFAP (green) and with antibodies against the four AMPA receptor subunits: (A, B) GluR1; (C, D) GluR2; (E, F) <t>GluR3</t> and (G, H) GluR4 (red). The images were acquired from rare regions with high density of glial cells to show that the GFAP-positive cells do not express AMPA receptors. Scale bar: 50 µm.
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    Image Search Results


    Journal: iScience

    Article Title: GluK2 Q/R editing regulates kainate receptor signaling and long-term potentiation of AMPA receptors

    doi: 10.1016/j.isci.2023.107708

    Figure Lengend Snippet:

    Article Snippet: Rabbit Polyclonal Anti-GluA3 , Alomone , RRID: AB_2039883.

    Techniques: Recombinant, Protein Extraction, Software

    Double labeling of rat cortical neurons at DIV5 and DIV26 (20×) with an antibody against the glial marker GFAP (green) and with antibodies against the four AMPA receptor subunits: (A, B) GluR1; (C, D) GluR2; (E, F) GluR3 and (G, H) GluR4 (red). The images were acquired from rare regions with high density of glial cells to show that the GFAP-positive cells do not express AMPA receptors. Scale bar: 50 µm.

    Journal: PLoS ONE

    Article Title: AMPA Receptor Regulation at the mRNA and Protein Level in Rat Primary Cortical Cultures

    doi: 10.1371/journal.pone.0025350

    Figure Lengend Snippet: Double labeling of rat cortical neurons at DIV5 and DIV26 (20×) with an antibody against the glial marker GFAP (green) and with antibodies against the four AMPA receptor subunits: (A, B) GluR1; (C, D) GluR2; (E, F) GluR3 and (G, H) GluR4 (red). The images were acquired from rare regions with high density of glial cells to show that the GFAP-positive cells do not express AMPA receptors. Scale bar: 50 µm.

    Article Snippet: The membranes were blocked for 60 min with 3% nonfat dry milk or 2% BSA in TBS-T (Tris-buffered saline with 0.1% Tween-20, Sigma-Aldrich) and then incubated overnight at 4°C in the blocking solution with the primary antibodies corresponding to either rabbit polyclonal anti-GluR1 (1∶200, Millipore), rabbit polyclonal anti-GluR2 (1∶2500, Millipore), rabbit polyclonal anti-GluR3 (1∶500, Alomone Labs Ltd) or rabbit polyclonal anti-GluR4 (1∶100, Millipore) with mouse monoclonal anti-GAPDH (1∶2500, Sigma-Aldrich) as a loading control.

    Techniques: Labeling, Marker